Open source software tools

FLIM data analysis software:  FLIMfit, our open source MATLAB-based software that provides a range of fitting techniques including global analysis. This will run on a standard personal computer or laptop but we recommend investing in at least 64 GB RAM and a  reasonable multicore processor if you intend to analyse large FLIM data sets. The FLIMfit software package is available as a client for the OMERO platform and can also be used as a stand-alone MATLAB application.

Wide-field time-gated FLIM data acquisition: our open source software, openFLIM-GOI, is a MicroManager plug-in and runs on a standard personal computer requiring ~8 GB RAM with USB and serial ports to interface with the equipment components.

FLIM high content analysis: our openFLIM-HCA software for automated multiwell plate wide-field time-gated FLIM (instrument control and data acquisition) using wide-field time-gated FLIM of samples arrayed in a 96 well plate is written in MicroManager.

Open instrumentation

The technology developed in this project will contribute to the openScopes initiative, that aims to provide the know-how and open source software tools to implement a suite of open source instrumentation for light microscopy and related modalities that are intended to enable researchers to implement, maintain and upgrade advanced optical imaging capabilities at relatively low cost, including in less affluent research communities where technical support may not be available. These capabilities include FLIM, optical projection tomography, high content analysis and super-resolved microscopy. In general they can be implemented as upgrades to existing commercial microscope frames or they can be constructed around the new, cost-effective, modular openFrame platform, for which CAD files may be downloaded for laboratories to fabricate their own components or they can be purchased commercially.

openFLIM hardware: For a list of components to assemble a wide-field time-gated FLIM microscope system or an optical sectioning wide-field FLIM microscope utilising a Nipkow spinning disc confocal scanner, please download the openFLIM hardware list.